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Recombinase Polymerase Amplification (RPA) Troubleshooting
Recombinase Polymerase Amplification (RPA) is an isothermal DNA amplification technique used to rapidly amplify specific DNA sequences without the need for thermal cycling, as in PCR. RPA works at a constant low temperature (typically 37-42°C) and uses a recombinase enzyme to bind primers to homologous sequences in the template DNA. Once bound ...
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